The list contains 23,614 full-length cDNA clones from the Japanese malting barley variety Haruna Nijo (Matsumoto et al
., 2011. Plant Physiol
. 156: 20-28). More information, including the expression profiles and physical locations on barley chromosomes (genomic sequences assembled by the International Barley Genome Sequencing Consortium, 2012. Nature
491: 711-716), can be obtained by accessing to our Barley Gene Expression Database (bex-db)
(Tanaka et al., 2013. Breeding Science
63: 430-434).Clones are distributed as plasmid DNAs. Vector is pFLCIII. DNA was extracted by 40 microL 10mM Tris (pH 8.6) and dried by evaporation. Dissolve with H2
O before transformation.